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Product Profile

Product Name	Anti-Annexin V antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	ANXA5
Gene Synonyms	PP4 ANX5 ENX2 RPRGL3 HEL-S-7 More
Gene Full Name	annexin A5
Gene Summary	The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications. [provided by RefSeq, Dec 2019] More
Molecular Weight(MW)	36 kDa
Source	Rabbit
Cellular Localizatio	Cytosol, collagen-containing extracellular matrix, endothelial microparticle, extracellular exosome, extracellular region, external side of plasma membrane, cytoplasm, focal adhesion, intracellular, membrane.

Application

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WB

Western blot analysis using Annexin V antibody against A431(1), PANC-1(2),NIH3T3(3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Annexin V antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using Annexin V antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.